› 1aqa

Solution NMR

SOLUTION STRUCTURE OF REDUCED MICROSOMAL RAT CYTOCHROME B5, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1aqa/pdb
PDB entry 1aqa coloured by chain, front view.
PDB entry 1aqa coloured by chain, side view.
PDB entry 1aqa coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Solution structure of reduced microsomal rat cytochrome b5.
Banci L, Bertini I, Ferroni F, Rosato A
Eur J Biochem 249 270-9 (1997)
PMID: 9363779

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.81 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00173 (Residues: 6-99; Coverage: 70%)
Gene names: Cyb5, Cyb5a
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE MOLECULAR DYNAMICS SIMULATED ANNEALING, RESTRAINED ENERGY MINIMIZATION
Expression system: Escherichia coli

Quick links

Citations

2 review citations

NMR solution structure, backbone mobility, and homology modeling of c-type cytochromes from gram-positive bacteria.
Banci et al. (2002)
1 more

2 mentions without citation

PGRMC1: An enigmatic heme-binding protein.
McGuire et al. (2023)
1 more