› 1as4

X-ray diffraction
2.1Å resolution

CLEAVED ANTICHYMOTRYPSIN A349R

Released:
DOI: 10.2210/pdb1as4/pdb
PDB entry 1as4 coloured by chain, front view.
PDB entry 1as4 coloured by chain, side view.
PDB entry 1as4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering an anion-binding cavity in antichymotrypsin modulates the "spring-loaded" serpin-protease interaction.
Lukacs CM, Rubin H, Christianson DW
Biochemistry 37 3297-304 (1998)
PMID: 9521649

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133792 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-1-antichymotrypsin His-Pro-less Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01011 (Residues: 43-383; Coverage: 85%)
Gene names: AACT, GIG24, GIG25, SERPINA3
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Alpha-1-antichymotrypsin His-Pro-less Chain: B
Molecule details ›
Chain: B
Length: 37 amino acids
Theoretical weight: 4.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01011 (Residues: 387-423; Coverage: 9%)
Gene names: AACT, GIG24, GIG25, SERPINA3

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 71.7Å b: 78.35Å c: 79.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.24
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New approaches to rational drug design.
Farber GK. (1999)
1 more

8 mentions without citation

Insufficiently dehydrated hydrogen bonds as determinants of protein interactions.
Fernández et al. (2003)
7 more