› 1asl

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURES OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE IN TWO CONFORMATIONS: COMPARISON OF AN UNLIGANDED OPEN AND TWO LIGANDED CLOSED FORMS

Released:
DOI: 10.2210/pdb1asl/pdb
PDB entry 1asl coloured by chain, front view.
PDB entry 1asl coloured by chain, side view.
PDB entry 1asl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of Escherichia coli aspartate aminotransferase in two conformations. Comparison of an unliganded open and two liganded closed forms.
Jäger J, Moser M, Sauder U, Jansonius JN
J Mol Biol 239 285-305 (1994)
PMID: 8196059

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PLA 2 x PLA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 85.6Å b: 78.8Å c: 89.6Å
α: 90° β: 118.6° γ: 90°
R-values:
R R work R free
0.183 0.183 not available
Expression system: Escherichia coli

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Citations

7 review citations

The manifold of vitamin B6 dependent enzymes.
Schneider et al. (2000)
6 more