› 1avq

X-ray diffraction
2.4Å resolution

TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS

Released:
DOI: 10.2210/pdb1avq/pdb
PDB entry 1avq coloured by chain, front view.
PDB entry 1avq coloured by chain, side view.
PDB entry 1avq coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
Toroidal structure of lambda-exonuclease.
Kovall R, Matthews BW
Science 277 1824-7 (1997)
PMID: 9295273

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exonuclease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 228 amino acids
Theoretical weight: 26.17 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03697 (Residues: 1-226; Coverage: 100%)
Gene names: exo, red-alpha, redX
Sequence domains: YqaJ-like viral recombinase domain
Structure domains: Lambda Exonuclease; Chain A

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41212
Unit cell:
a: 156.7Å b: 156.7Å c: 131.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 not available
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.
Kuzminov A. (1999)
21 more

16 mentions without citation

Mechanical design of translocating motor proteins.
Hwang et al. (2009)
15 more