› 1avz

X-ray diffraction
3Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136957 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-terminal core protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17.57 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03406 (Residues: 58-206; Coverage: 72%)
Gene name: nef
Sequence domains: Negative factor, (F-Protein) or Nef
Structure domains: Nef Regulatory Factor
Tyrosine-protein kinase Fyn Chain: C
Molecule details ›
Chain: C
Length: 57 amino acids
Theoretical weight: 6.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 85-141; Coverage: 11%)
Gene name: FYN
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P6522
Unit cell:
a: 108.2Å b: 108.2Å c: 223.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Structure--function relationships in HIV-1 Nef.
Geyer et al. (2001)
12 more

42 mentions without citation

Mechanisms of HIV-1 Nef function and intracellular signaling.
Foster et al. (2011)
41 more