› 1azt

X-ray diffraction
2.3Å resolution

GS-ALPHA COMPLEXED WITH GTP-GAMMA-S

Released:
DOI: 10.2210/pdb1azt/pdb
PDB entry 1azt coloured by chain, front view.
PDB entry 1azt coloured by chain, side view.
PDB entry 1azt coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of the adenylyl cyclase activator Gsalpha.
Sunahara RK, Tesmer JJ, Gilman AG, Sprang SR
Science 278 1943-7 (1997)
PMID: 9395396

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 46.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand PO4 16 x PO4
Ligand GSP 2 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 88.3Å b: 96.5Å c: 133.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

The regulator of G protein signaling family.
De Vries et al. (2000)
26 more

64 mentions without citation

Structure and dynamics of GPCR signaling complexes.
Hilger et al. (2018)
63 more