› 1b24

X-ray diffraction
2.2Å resolution

I-DMOI, INTRON-ENCODED ENDONUCLEASE

Released:
DOI: 10.2210/pdb1b24/pdb
PDB entry 1b24 coloured by chain, front view.
PDB entry 1b24 coloured by chain, side view.
PDB entry 1b24 coloured by chain, top view.
Source organism: Desulfurococcus mucosus
Primary publication:
Crystal structure of the thermostable archaeal intron-encoded endonuclease I-DmoI.
Silva GH, Dalgaard JZ, Belfort M, Van Roey P
J Mol Biol 286 1123-36 (1999)
PMID: 10047486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homing endonuclease I-DmoI Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 22.18 KDa
Source organism: Desulfurococcus mucosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21505 (Residues: 1-188; Coverage: 97%)
Sequence domains: LAGLIDADG-like domain
Structure domains: Homing endonucleases

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 93.73Å b: 37.22Å c: 56.28Å
α: 90° β: 112.8° γ: 90°
R-values:
R R work R free
0.218 0.218 0.324
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
7 more

4 mentions without citation

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.
Epinat et al. (2003)
3 more