› 1b2e

X-ray diffraction
1.9Å resolution

PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.50 COORDINATES)

Released:
DOI: 10.2210/pdb1b2e/pdb
PDB entry 1b2e coloured by chain, front view.
PDB entry 1b2e coloured by chain, side view.
PDB entry 1b2e coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Crystallographic titration of cubic insulin crystals: pH affects GluB13 switching and sulfate binding.
Diao J
Acta Crystallogr D Biol Crystallogr 59 670-6 (2003)
PMID: 12657786

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexamer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134237 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I213
Unit cell:
a: 78.744Å b: 78.744Å c: 78.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.229

Quick links

Citations

1 review citation

Intracellular pH sensors: design principles and functional significance.
Srivastava et al. (2007)
7 other articles

1 mention without citation

Novel covalently linked insulin dimer engineered to investigate the function of insulin dimerization.
Vinther et al. (2012)