› 1b4e

X-ray diffraction
2Å resolution

X-ray structure of 5-aminolevulinic acid dehydratase complexed with the inhibitor levulinic acid

Released:
DOI: 10.2210/pdb1b4e/pdb
PDB entry 1b4e coloured by chain, front view.
PDB entry 1b4e coloured by chain, side view.
PDB entry 1b4e coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
X-ray structure of 5-aminolevulinic acid dehydratase from Escherichia coli complexed with the inhibitor levulinic acid at 2.0 A resolution.
Erskine PT, Norton E, Cooper JB, Lambert R, Coker A, Lewis G, Spencer P, Sarwar M, Wood SP, Warren MJ, Shoolingin-Jordan PM
Biochemistry 38 4266-76 (1999)
PMID: 10194344

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACB2 (Residues: 2-324; Coverage: 100%)
Gene names: JW0361, b0369, hemB, ncf
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand SO4 3 x SO4
Ligand ZN 3 x ZN
Ligand SHF 1 x SHF
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: I422
Unit cell:
a: 126.7Å b: 126.7Å c: 141.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.255
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Biosynthesis of heme in mammals.
Ajioka et al. (2006)
9 more

1 mention without citation

Crystal structure of Toxoplasma gondii porphobilinogen synthase: insights on octameric structure and porphobilinogen formation.
Jaffe et al. (2011)