› 1b57

X-ray diffraction
2Å resolution

CLASS II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE IN COMPLEX WITH PHOSPHOGLYCOLOHYDROXAMATE

Released:
DOI: 10.2210/pdb1b57/pdb
PDB entry 1b57 coloured by chain, front view.
PDB entry 1b57 coloured by chain, side view.
PDB entry 1b57 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of Escherichia coli class II fructose-1, 6-bisphosphate aldolase in complex with phosphoglycolohydroxamate reveals details of mechanism and specificity.
Hall DR, Leonard GA, Reed CD, Watt CI, Berry A, Hunter WN
J Mol Biol 287 383-94 (1999)
PMID: 10080900

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-bisphosphate aldolase class 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 39.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB71 (Residues: 2-359; Coverage: 100%)
Gene names: JW2892, b2925, fba, fbaA, fda
Sequence domains: Fructose-bisphosphate aldolase class-II
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand ZN 7 x ZN
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand PGH 2 x PGH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3121
Unit cell:
a: 78.237Å b: 78.237Å c: 289.687Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.23
Expression system: Escherichia coli

Quick links

Citations

9 review citations

One fold with many functions: the evolutionary relationships between TIM barrel families based on their sequences, structures and functions.
Nagano et al. (2002)
8 more

24 mentions without citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
23 more