› 1b5b

Solution NMR

RAT FERROCYTOCHROME B5 B CONFORMATION, NMR, 1 STRUCTURE

Released:
DOI: 10.2210/pdb1b5b/pdb
PDB entry 1b5b coloured by chain, front view.
PDB entry 1b5b coloured by chain, side view.
PDB entry 1b5b coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The origin of differences in the physical properties of the equilibrium forms of cytochrome b5 revealed through high-resolution NMR structures and backbone dynamic analyses.
Dangi B, Sarma S, Yan C, Banville DL, Guiles RD
Biochemistry 37 8289-302 (1998)
PMID: 9622481

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.81 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00173 (Residues: 6-99; Coverage: 70%)
Gene names: Cyb5, Cyb5a
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Diversity and conservation of interactions for binding heme in b-type heme proteins.
Schneider et al. (2007)
15 other articles

1 mention without citation

A cell-based assay for RNA synthesis by the HCV polymerase reveals new insights on mechanism of polymerase inhibitors and modulation by NS5A.
Ranjith-Kumar et al. (2011)