› 1b7f

X-ray diffraction
2.6Å resolution

SXL-LETHAL PROTEIN/RNA COMPLEX

Released:
DOI: 10.2210/pdb1b7f/pdb
PDB entry 1b7f coloured by chain, front view.
PDB entry 1b7f coloured by chain, side view.
PDB entry 1b7f coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein.
Handa N, Nureki O, Kurimoto K, Kim I, Sakamoto H, Shimura Y, Muto Y, Yokoyama S
Nature 398 579-85 (1999)
PMID: 10217141

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148561 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein sex-lethal Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 18.97 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19339 (Residues: 122-289; Coverage: 48%)
Gene names: CG43770, Sx1, Sxl
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(P*GP*UP*UP*GP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 12 nucleotides
Theoretical weight: 3.71 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 77.9Å b: 86.8Å c: 160.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

26 review citations

Mechanisms of alternative pre-messenger RNA splicing.
Black DL. (2003)
25 more

33 mentions without citation

Sequence-specific binding of single-stranded RNA: is there a code for recognition?
Auweter et al. (2006)
32 more