› 1b8x

X-ray diffraction
2.7Å resolution

GLUTATHIONE S-TRANSFERASE FUSED WITH THE NUCLEAR MATRIX TARGETING SIGNAL OF THE TRANSCRIPTION FACTOR AML-1

Released:
DOI: 10.2210/pdb1b8x/pdb
PDB entry 1b8x coloured by chain, front view.
PDB entry 1b8x coloured by chain, side view.
PDB entry 1b8x coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML-1/CBF-alpha2.
Tang L, Guo B, van Wijnen AJ, Lian JB, Stein JL, Stein GS, Zhou GW
J Struct Biol 123 83-5 (1998)
PMID: 9774548

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase class-mu 26 kDa isozyme Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 32.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08515 (Residues: 2-218; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P43212
Unit cell:
a: 93.4Å b: 93.4Å c: 57.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.31
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structural analysis of regulatory protein domains using GST-fusion proteins.
Zhan et al. (2001)
10 more

3 mentions without citation

Principles and characteristics of biological assemblies in experimentally determined protein structures.
Xu et al. (2019)
2 more