› 1ba3

X-ray diffraction
2.2Å resolution

FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM

Released:
DOI: 10.2210/pdb1ba3/pdb
PDB entry 1ba3 coloured by chain, front view.
PDB entry 1ba3 coloured by chain, side view.
PDB entry 1ba3 coloured by chain, top view.
Source organism: Photinus pyralis
Primary publication:
Structural basis for the inhibition of firefly luciferase by a general anesthetic.
Franks NP, Jenkins A, Conti E, Lieb WR, Brick P
Biophys J 75 2205-11 (1998)
PMID: 9788915

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.82 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MBR 2 x MBR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P41212
Unit cell:
a: 119.56Å b: 119.56Å c: 95.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.239
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Mechanisms of actions of inhaled anesthetics.
Campagna et al. (2003)
17 more

14 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
13 more