Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Capsid protein p24 (preferred)
PDBe Complex ID:
PDB-CPX-146209 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein p24
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.11 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Gag protein p24 C-terminal domain
Structure domains: Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Length: 101 amino acids
Theoretical weight: 11.11 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P12497 (Residues: 277-377; Coverage: 7%)
Sequence domains: Gag protein p24 C-terminal domain
Structure domains: Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X8C
Spacegroup:
I41
Expression system: Escherichia coli BL21(DE3)
