› 1bc7

X-ray diffraction
2.01Å resolution

SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX

Released:
DOI: 10.2210/pdb1bc7/pdb
PDB entry 1bc7 coloured by chain, front view.
PDB entry 1bc7 coloured by chain, side view.
PDB entry 1bc7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
Mo Y, Vaessen B, Johnston K, Marmorstein R
Mol Cell 2 201-12 (1998)
PMID: 9734357

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113472 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS domain-containing protein Elk-4 Chain: C
Molecule details ›
Chain: C
Length: 93 amino acids
Theoretical weight: 11.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28324 (Residues: 1-93; Coverage: 22%)
Gene names: ELK4, SAP1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*CP*AP*GP*GP*AP*TP*GP*TP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 11 nucleotides
Theoretical weight: 3.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*AP*CP*AP*TP*CP*CP*TP*GP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.27 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 34.503Å b: 62.306Å c: 38.865Å
α: 90° β: 107.17° γ: 90°
R-values:
R R work R free
0.222 0.222 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Genomic and biochemical insights into the specificity of ETS transcription factors.
Hollenhorst et al. (2011)
8 more

8 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
7 more