› 1bc8

X-ray diffraction
1.93Å resolution

STRUCTURES OF SAP-1 BOUND TO DNA SEQUENCES FROM THE E74 AND C-FOS PROMOTERS PROVIDE INSIGHTS INTO HOW ETS PROTEINS DISCRIMINATE BETWEEN RELATED DNA TARGETS

Released:
DOI: 10.2210/pdb1bc8/pdb
PDB entry 1bc8 coloured by chain, front view.
PDB entry 1bc8 coloured by chain, side view.
PDB entry 1bc8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
Mo Y, Vaessen B, Johnston K, Marmorstein R
Mol Cell 2 201-12 (1998)
PMID: 9734357

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113473 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS domain-containing protein Elk-4 Chain: C
Molecule details ›
Chain: C
Length: 93 amino acids
Theoretical weight: 11.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28324 (Residues: 1-93; Coverage: 22%)
Gene names: ELK4, SAP1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*TP*AP*CP*CP*GP*GP*AP*AP*GP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.07 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 31.59Å b: 55.67Å c: 44.23Å
α: 90° β: 107.16° γ: 90°
R-values:
R R work R free
0.22 0.22 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Genomic and biochemical insights into the specificity of ETS transcription factors.
Hollenhorst et al. (2011)
8 more

23 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
22 more