› 1bd2

X-ray diffraction
2.5Å resolution

COMPLEX BETWEEN HUMAN T-CELL RECEPTOR B7, VIRAL PEPTIDE (TAX) AND MHC CLASS I MOLECULE HLA-A 0201

Released:
DOI: 10.2210/pdb1bd2/pdb
PDB entry 1bd2 coloured by chain, front view.
PDB entry 1bd2 coloured by chain, side view.
PDB entry 1bd2 coloured by chain, top view.
Source organisms:
Primary publication:
Two human T cell receptors bind in a similar diagonal mode to the HLA-A2/Tax peptide complex using different TCR amino acids.
Ding YH, Smith KJ, Garboczi DN, Utz U, Biddison WE, Wiley DC
Immunity 8 403-11 (1998)
PMID: 9586631

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-102288 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Tax-1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell leukemia virus type I
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax
T cell receptor alpha chain constant; T cell receptor alpha variable 29/delta variable 5 Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04437 (Residues: 28-118; Coverage: 93%)
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC, TRAV29DV5
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta variable 6-5 Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K0K1A5 (Residues: 20-114; Coverage: 100%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 63.8Å b: 73.3Å c: 217.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.312
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

66 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
65 more

50 mentions without citation

Evolutionarily conserved amino acids that control TCR-MHC interaction.
Marrack et al. (2008)
49 more