› 1bdi

X-ray diffraction
3Å resolution

PURINE REPRESSOR MUTANT-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX

Released:
DOI: 10.2210/pdb1bdi/pdb
PDB entry 1bdi coloured by chain, front view.
PDB entry 1bdi coloured by chain, side view.
PDB entry 1bdi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A Positively Charged Residue Bound in the Minor Groove Does not Alter the Bending of a DNA Duplex
Glasfeld A, Schumacher MA, Choi KY, Zalkin H, Brennan RG
J. Am. Chem. Soc. 118 13073-13074 (1996)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113478 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional repressor PurR Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.09 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ACP7 (Residues: 2-341; Coverage: 100%)
Gene names: JW1650, b1658, purR
Sequence domains:
Structure domains:
DNA (5'-D(*TP*AP*CP*GP*CP*AP*AP*AP*CP*GP*TP*TP*TP*GP*CP*GP*T )-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand HPA 1 x HPA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 176.16Å b: 94.99Å c: 81.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 not available not available
Expression system: Not provided

Quick links

Citations

1 mention without citation

Local combinational variables: an approach used in DNA-binding helix-turn-helix motif prediction with sequence information.
Xiong et al. (2009)