› 1bdv

X-ray diffraction
2.8Å resolution

ARC FV10 COCRYSTAL

Released:
DOI: 10.2210/pdb1bdv/pdb
PDB entry 1bdv coloured by chain, front view.
PDB entry 1bdv coloured by chain, side view.
PDB entry 1bdv coloured by chain, top view.
Source organism: Lederbergvirus P22
Primary publication:
Origins of DNA-binding specificity: role of protein contacts with the DNA backbone.
Schildbach JF, Karzai AW, Raumann BE, Sauer RT
Proc Natl Acad Sci U S A 96 811-7 (1999)
PMID: 9927650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113481 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional repressor arc Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 53 amino acids
Theoretical weight: 6.19 KDa
Source organism: Salmonella virus P22
Expression system: Escherichia coli
UniProt:
  • Canonical: P03050 (Residues: 1-53; Coverage: 100%)
Gene name: arc
Sequence domains: Arc-like DNA binding domain
Structure domains: Met repressor-like
DNA (5'-D(*TP*AP*TP*AP*GP*TP*AP*GP*AP*GP*TP*GP*CP*TP*TP*CP*TP*AP*TP*CP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 22 nucleotides
Theoretical weight: 6.76 KDa
Source organism: Lederbergvirus P22
Expression system: Not provided
DNA (5'-D(*AP*AP*TP*GP*AP*TP*AP*GP*AP*AP*GP*CP*AP*CP*TP*CP*TP*AP*CP*TP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 22 nucleotides
Theoretical weight: 6.74 KDa
Source organism: Lederbergvirus P22
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 62.84Å b: 56.31Å c: 52.98Å
α: 90° β: 105.03° γ: 90°
R-values:
R R work R free
0.244 0.244 0.301
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Thermodynamic and kinetic analyses for understanding sequence-specific DNA recognition.
Oda et al. (2000)
39 other articles

3 mentions without citation

Origins of DNA-binding specificity: role of protein contacts with the DNA backbone.
Schildbach et al. (1999)
2 more