› 1bdx

X-ray diffraction
6Å resolution

E. COLI DNA HELICASE RUVA WITH BOUND DNA HOLLIDAY JUNCTION, ALPHA CARBONS AND PHOSPHATE ATOMS ONLY

Released:
DOI: 10.2210/pdb1bdx/pdb
PDB entry 1bdx coloured by chain, front view.
PDB entry 1bdx coloured by chain, side view.
PDB entry 1bdx coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Crystal structure of E.coli RuvA with bound DNA Holliday junction at 6 A resolution.
Hargreaves D, Rice DW, Sedelnikova SE, Artymiuk PJ, Lloyd RG, Rafferty JB
Nat Struct Biol 5 441-6 (1998)
PMID: 9628481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113482 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Holliday junction branch migration complex subunit RuvA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 203 amino acids
Theoretical weight: 22.11 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A809 (Residues: 1-203; Coverage: 100%)
Gene names: JW1850, b1861, ruvA
Sequence domains:
DNA (5'-D(P*GP*CP*AP*TP*GP*CP*AP*TP*AP*TP*GP*CP*AP*TP*GP*C)-3') Chains: J, K, L, M
Molecule details ›
Chains: J, K, L, M
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 148Å b: 148Å c: 105.6Å
α: 90° β: 123° γ: 90°
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

20 review citations

Molecular views of recombination proteins and their control.
West SC. (2003)
19 more

2 mentions without citation

Common fold in helix-hairpin-helix proteins.
Shao et al. (2000)
1 more