› 1bf4

X-ray diffraction
1.6Å resolution

CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX

Released:
DOI: 10.2210/pdb1bf4/pdb
PDB entry 1bf4 coloured by chain, front view.
PDB entry 1bf4 coloured by chain, side view.
PDB entry 1bf4 coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA.
Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary BS, Edmondson SP, Shriver JW, Wang AH
Nat Struct Biol 5 782-6 (1998)
PMID: 9731772

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113485 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein 7d Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.16 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: P13123 (Residues: 4-38, 39-66; Coverage: 91%)
Gene name: Saci_0064
Sequence domains: 7kD DNA-binding domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
DNA (5'-D(*GP*CP*GP*TP*5IUP*CP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.53 KDa
DNA (5'-D(*GP*CP*GP*AP*AP*CP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.44 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5IU 1 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 46.87Å b: 49.67Å c: 37.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.288
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Holding it together: chromatin in the Archaea.
White et al. (2002)
15 more

13 mentions without citation

Archaea: The Final Frontier of Chromatin.
Laursen et al. (2021)
12 more