› 1bfp

X-ray diffraction
2.1Å resolution

BLUE VARIANT OF GREEN FLUORESCENT PROTEIN

Released:
DOI: 10.2210/pdb1bfp/pdb
PDB entry 1bfp coloured by chain, front view.
PDB entry 1bfp coloured by chain, side view.
PDB entry 1bfp coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Crystal structure and photodynamic behavior of the blue emission variant Y66H/Y145F of green fluorescent protein.
Wachter RM, King BA, Heim R, Kallio K, Tsien RY, Boxer SG, Remington SJ
Biochemistry 36 9759-65 (1997)
PMID: 9245407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.89 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand IIC 1 x IIC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 52.01Å b: 63.09Å c: 69.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The green fluorescent protein.
Tsien RY. (1998)
12 more

7 mentions without citation

Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application.
Jäger et al. (2020)
6 more