› 1bg7

X-ray diffraction
1.85Å resolution

LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?

Released:
DOI: 10.2210/pdb1bg7/pdb
PDB entry 1bg7 coloured by chain, front view.
PDB entry 1bg7 coloured by chain, side view.
PDB entry 1bg7 coloured by chain, top view.
Source organism: Lithobates catesbeianus
Primary publication:
Localized unfolding at the junction of three ferritin subunits. A mechanism for iron release?
Takagi H, Shi D, Ha Y, Allewell NM, Theil EC
J Biol Chem 273 18685-8 (1998)
PMID: 9668036

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin, higher subunit Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.55 KDa
Source organism: Lithobates catesbeianus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07229 (Residues: 1-176; Coverage: 100%)
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: F432
Unit cell:
a: 182.8Å b: 182.8Å c: 182.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Regulation of cellular iron metabolism.
Wang et al. (2011)
19 more

5 mentions without citation

Ferritin protein nanocages use ion channels, catalytic sites, and nucleation channels to manage iron/oxygen chemistry.
Theil EC. (2011)
4 more