› 1bhw

X-ray diffraction
4.1Å resolution

LOW TEMPERATURE MIDDLE RESOLUTION STRUCTURE OF XYLOSE ISOMERASE FROM MASC DATA

Released:
DOI: 10.2210/pdb1bhw/pdb
PDB entry 1bhw coloured by chain, front view.
PDB entry 1bhw coloured by chain, side view.
PDB entry 1bhw coloured by chain, top view.
Source organism: Actinoplanes missouriensis
Primary publication:
Multiwavelength anomalous solvent contrast (MASC): derivation of envelope structure-factor amplitudes and comparison with model values.
Ramin M, Shepard W, Fourme R, Kahn R
Acta Crystallogr D Biol Crystallogr 55 157-67 (1999)
PMID: 10089406

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 43.4 KDa
Source organism: Actinoplanes missouriensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P12851 (Residues: 2-394; Coverage: 100%)
Gene names: AMIS_10350, XI, xylA
Sequence domains: Xylose isomerase-like TIM barrel

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P3221
Unit cell:
a: 141.91Å b: 141.91Å c: 227.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.315 0.315 0.321
Expression system: Escherichia coli

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Citations

5 citation in other articles

Structure and function of proteins in hydrated choline dihydrogen phosphate ionic liquid.
Weaver et al. (2012)
4 more