› 1bjo

X-ray diffraction
2.8Å resolution

THE STRUCTURE OF PHOSPHOSERINE AMINOTRANSFERASE FROM E. COLI IN COMPLEX WITH ALPHA-METHYL-L-GLUTAMATE

Released:
DOI: 10.2210/pdb1bjo/pdb
PDB entry 1bjo coloured by chain, front view.
PDB entry 1bjo coloured by chain, side view.
PDB entry 1bjo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of phosphoserine aminotransferase from Escherichia coli at 2.3 A resolution: comparison of the unligated enzyme and a complex with alpha-methyl-l-glutamate.
Hester G, Stark W, Moser M, Kallen J, Marković-Housley Z, Jansonius JN
J Mol Biol 286 829-50 (1999)
PMID: 10024454

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150049 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphoserine aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 39.71 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P23721 (Residues: 3-362; Coverage: 99%)
Gene names: JW0890, b0907, pdxC, pdxF, serC
Sequence domains: Aminotransferase class-V
Structure domains:
Phosphoserine aminotransferase Chain: B
Molecule details ›
Chain: B
Length: 360 amino acids
Theoretical weight: 39.94 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P23721 (Residues: 3-362; Coverage: 99%)
Gene names: JW0890, b0907, pdxC, pdxF, serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand GAM 1 x GAM
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-20
Spacegroup: P212121
Unit cell:
a: 68.86Å b: 94.45Å c: 131.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.251
Expression system: Not provided

Quick links

Citations

3 review citations

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
2 more

8 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
7 more