› 1bmb

X-ray diffraction
1.8Å resolution

GRB2-SH2 DOMAIN IN COMPLEX WITH KPFY*VNVEF (PKF270-974)

Released:
DOI: 10.2210/pdb1bmb/pdb
PDB entry 1bmb coloured by chain, front view.
PDB entry 1bmb coloured by chain, side view.
PDB entry 1bmb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and conformational requirements for high-affinity binding to the SH2 domain of Grb2(1).
Ettmayer P, France D, Gounarides J, Jarosinski M, Martin MS, Rondeau JM, Sabio M, Topiol S, Weidmann B, Zurini M, Bair KW
J Med Chem 42 971-80 (1999)
PMID: 10090780

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-158854 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 14.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62993 (Residues: 49-168; Coverage: 55%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SH2 domain
PROTEIN (PKF270-974) Chain: I
Molecule details ›
Chain: I
Length: 9 amino acids
Theoretical weight: 1.22 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P41212
Unit cell:
a: 51.13Å b: 51.13Å c: 90.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.218
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Correlating structure and energetics in protein-ligand interactions: paradigms and paradoxes.
Martin et al. (2013)
5 more

14 mentions without citation

Molecular mechanisms of SH2- and PTB-domain-containing proteins in receptor tyrosine kinase signaling.
Wagner et al. (2013)
13 more