› 1boo

X-ray diffraction
2.8Å resolution

PVUII DNA METHYLTRANSFERASE (CYTOSINE-N4-SPECIFIC)

Released:
DOI: 10.2210/pdb1boo/pdb
PDB entry 1boo coloured by chain, front view.
PDB entry 1boo coloured by chain, side view.
PDB entry 1boo coloured by chain, top view.
Source organism: Proteus vulgaris
Primary publication:
Structure of pvu II DNA-(cytosine N4) methyltransferase, an example of domain permutation and protein fold assignment.
Gong W, O'Gara M, Blumenthal RM, Cheng X
Nucleic Acids Res 25 2702-15 (1997)
PMID: 9207015

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II methyltransferase M.PvuII Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.96 KDa
Source organism: Proteus vulgaris
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P11409 (Residues: 14-336; Coverage: 96%)
Gene name: pvuIIM
Sequence domains: DNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 48.8Å b: 112.4Å c: 59.3Å
α: 90° β: 109.2° γ: 90°
R-values:
R R work R free
0.193 0.193 0.283
Expression system: Escherichia coli str. K-12 substr. DH10B

Quick links

Citations

15 review citations

Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases.
Jeltsch A. (2002)
14 more

16 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
15 more