› 1bp7

X-ray diffraction
3Å resolution

GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

Released:
DOI: 10.2210/pdb1bp7/pdb
PDB entry 1bp7 coloured by chain, front view.
PDB entry 1bp7 coloured by chain, side view.
PDB entry 1bp7 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.
Jurica MS, Monnat RJ, Stoddard BL
Mol Cell 2 469-76 (1998)
PMID: 9809068

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113499 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.52 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP* CP*G)-3') Chains: 1, 3
Molecule details ›
Chains: 1, 3
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
DNA (5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP* GP*C)-3') Chains: 2, 4
Molecule details ›
Chains: 2, 4
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 67.556Å b: 89.259Å c: 177.516Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.281
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
18 more

5 mentions without citation

Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases.
Lucas et al. (2001)
4 more