› 1bpb

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF RAT DNA POLYMERASE BETA: EVIDENCE FOR A COMMON POLYMERASE MECHANISM

Released:
DOI: 10.2210/pdb1bpb/pdb
PDB entry 1bpb coloured by chain, front view.
PDB entry 1bpb coloured by chain, side view.
PDB entry 1bpb coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism.
Sawaya MR, Pelletier H, Kumar A, Wilson SH, Kraut J
Science 264 1930-5 (1994)
PMID: 7516581

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.77 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P06766 (Residues: 88-335; Coverage: 74%)
Gene name: Polb
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 120.576Å b: 63.52Å c: 38.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 not available not available
Expression system: Not provided

Quick links

Citations

42 review citations

Eukaryotic DNA polymerases.
Hubscher et al. (2002)
41 more

6 mentions without citation

Structure and function relationships in mammalian DNA polymerases.
Hoitsma et al. (2020)
5 more