› 1bph

X-ray diffraction
2Å resolution

CONFORMATIONAL CHANGES IN CUBIC INSULIN CRYSTALS IN THE PH RANGE 7-11

Released:
DOI: 10.2210/pdb1bph/pdb
PDB entry 1bph coloured by chain, front view.
PDB entry 1bph coloured by chain, side view.
PDB entry 1bph coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Conformational changes in cubic insulin crystals in the pH range 7-11.
Gursky O, Badger J, Li Y, Caspar DL
Biophys J 63 1210-20 (1992)
PMID: 1477273

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134251 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.34 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P01317 (Residues: 85-105; Coverage: 26%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P01317 (Residues: 25-54; Coverage: 37%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand DCE 1 x DCE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I213
Unit cell:
a: 78.9Å b: 78.9Å c: 78.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 not available not available
Expression system: Not provided

Quick links

Citations

5 review citations

Intracellular pH sensors: design principles and functional significance.
Srivastava et al. (2007)
4 more

10 mentions without citation

Classification and energetics of the base-phosphate interactions in RNA.
Zirbel et al. (2009)
9 more