› 1bqb

X-ray diffraction
1.72Å resolution

AUREOLYSIN, STAPHYLOCOCCUS AUREUS METALLOPROTEINASE

Released:
DOI: 10.2210/pdb1bqb/pdb
PDB entry 1bqb coloured by chain, front view.
PDB entry 1bqb coloured by chain, side view.
PDB entry 1bqb coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Amino-acid sequence and three-dimensional structure of the Staphylococcus aureus metalloproteinase at 1.72 A resolution.
Banbula A, Potempa J, Travis J, Fernandez-Catalán C, Mann K, Huber R, Bode W, Medrano F
Structure 6 1185-93 (1998)
PMID: 9753696

Function and Biology Details

Reaction catalysed: 
Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residue. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc metalloproteinase aureolysin Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.36 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: P81177 (Residues: 209-509; Coverage: 62%)
Gene name: aur
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 33.7Å b: 79.5Å c: 100.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.239

Quick links

Citations

10 review citations

Structural aspects of the metzincin clan of metalloendopeptidases.
Gomis-Rüth FX. (2003)
9 more

11 mentions without citation

An Introduction to Bacterial Biofilms and Their Proteases, and Their Roles in Host Infection and Immune Evasion.
Ramírez-Larrota et al. (2022)
10 more