› 1bqh

X-ray diffraction
2.8Å resolution

MURINE CD8AA ECTODOMAIN FRAGMENT IN COMPLEX WITH H-2KB/VSV8

Released:
DOI: 10.2210/pdb1bqh/pdb
PDB entry 1bqh coloured by chain, front view.
PDB entry 1bqh coloured by chain, side view.
PDB entry 1bqh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8alphaalpha ectodomain fragment in complex with H-2Kb.
Kern PS, Teng MK, Smolyar A, Liu JH, Liu J, Hussey RE, Spoerl R, Chang HC, Reinherz EL, Wang JH
Immunity 9 519-30 (1998)
PMID: 9806638

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134573 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 274 amino acids
Theoretical weight: 31.65 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
PROTEIN (VSV8) Chains: C, F
Molecule details ›
Chains: C, F
Length: 8 amino acids
Theoretical weight: 956 Da
T-cell surface glycoprotein CD8 alpha chain Chains: G, H, I, K
Molecule details ›
Chains: G, H, I, K
Length: 129 amino acids
Theoretical weight: 14.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P01731 (Residues: 28-156; Coverage: 59%)
Gene names: Cd8a, Lyt-2, Lyt2
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 85.9Å b: 98.4Å c: 170.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.296
Expression system: Mus musculus

Quick links

Citations

34 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
33 more

14 mentions without citation

The structural basis of αβ T-lineage immune recognition: TCR docking topologies, mechanotransduction, and co-receptor function.
Wang et al. (2012)
13 more