› 1br3

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME

Released:
DOI: 10.2210/pdb1br3/pdb
PDB entry 1br3 coloured by chain, front view.
PDB entry 1br3 coloured by chain, side view.
PDB entry 1br3 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme.
Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF
Nat Struct Biol 6 151-6 (1999)
PMID: 10048927

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-113506 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DNA (10-23 DNA ENZYME) Chain: B
Molecule details ›
Chain: B
Length: 28 nucleotides
Theoretical weight: 8.51 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*AP*CP*AP*GP*AP*UP*GP*GP*GP*AP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 4.3 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P6122
Unit cell:
a: 63.45Å b: 63.45Å c: 216.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.251
Expression system: Not provided

Quick links

Citations

26 review citations

How RNA folds.
Tinoco et al. (1999)
25 more

3 mentions without citation

Biochemical and Biophysical Understanding of Metal Ion Selectivity of DNAzymes.
Hwang et al. (2016)
2 more