› 1brw

X-ray diffraction
2.1Å resolution

THE CRYSTAL STRUCTURE OF PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE IN A CLOSED CONFORMATION

Released:
DOI: 10.2210/pdb1brw/pdb
PDB entry 1brw coloured by chain, front view.
PDB entry 1brw coloured by chain, side view.
PDB entry 1brw coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.
Pugmire MJ, Ealick SE
Structure 6 1467-79 (1998)
PMID: 9817849

Function and Biology Details

Reaction catalysed: 
2'-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

4 bound ligands:
Ligand PO4 2 x PO4
Ligand CA 2 x CA
Ligand MES 2 x MES
Ligand URA 1 x URA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 53.57Å b: 70.45Å c: 122.78Å
α: 90° β: 98.02° γ: 90°
R-values:
R R work R free
0.232 0.232 0.276

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Citations

3 review citations

Structural analyses reveal two distinct families of nucleoside phosphorylases.
Pugmire et al. (2002)
2 more

10 mentions without citation

The Prodigal Compound: Return of Ribosyl 1,5-Bisphosphate as an Important Player in Metabolism.
Hove-Jensen et al. (2019)
9 more