› 1bsj

X-ray diffraction
3Å resolution

COBALT DEFORMYLASE INHIBITOR COMPLEX FROM E.COLI

Released:
DOI: 10.2210/pdb1bsj/pdb
PDB entry 1bsj coloured by chain, front view.
PDB entry 1bsj coloured by chain, side view.
PDB entry 1bsj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for the design of antibiotics targeting peptide deformylase.
Hao B, Gong W, Rajagopalan PT, Zhou Y, Pei D, Chan MK
Biochemistry 38 4712-9 (1999)
PMID: 10200158

Function and Biology Details

Reaction catalysed: 
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.23 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A6K3 (Residues: 2-169; Coverage: 99%)
Gene names: JW3248, b3287, def, fms
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

3 bound ligands:
Ligand CO 1 x CO
Ligand PO4 1 x PO4
Ligand MLN 1 x MLN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6522
Unit cell:
a: 99.08Å b: 99.08Å c: 111.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.168 0.207

Quick links

Citations

3 review citations

Peptide deformylase as a target for new generation, broad spectrum antimicrobial agents.
Giglione et al. (2000)
2 more