› 1bt1

X-ray diffraction
2.7Å resolution

CATECHOL OXIDASE FROM IPOMOEA BATATAS (SWEET POTATOES) IN THE NATIVE CU(II)-CU(II) STATE

Released:
DOI: 10.2210/pdb1bt1/pdb
PDB entry 1bt1 coloured by chain, front view.
PDB entry 1bt1 coloured by chain, side view.
PDB entry 1bt1 coloured by chain, top view.
Source organism: Ipomoea batatas
Primary publication:
Crystal structure of a plant catechol oxidase containing a dicopper center.
Klabunde T, Eicken C, Sacchettini JC, Krebs B
Nat Struct Biol 5 1084-90 (1998)
PMID: 9846879

Function and Biology Details

Reaction catalysed: 
2 catechol + O(2) = 2 1,2-benzoquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphenol oxidase I, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 38.81 KDa
Source organism: Ipomoea batatas
UniProt:
  • Canonical: Q9ZP19 (Residues: 1-345; Coverage: 70%)
Gene name: co-1
Sequence domains:
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

1 bound ligand:
Ligand C2O 2 x C2O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 45.82Å b: 164.75Å c: 52.16Å
α: 90° β: 97.5° γ: 90°
R-values:
R R work R free
0.168 0.168 0.256

Quick links

Citations

39 review citations

Tyrosinase/catecholoxidase activity of hemocyanins: structural basis and molecular mechanism.
Decker et al. (2000)
38 more

13 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
12 more