› 1buw

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF S-NITROSO-NITROSYL HUMAN HEMOGLOBIN A

Released:
DOI: 10.2210/pdb1buw/pdb
PDB entry 1buw coloured by chain, front view.
PDB entry 1buw coloured by chain, side view.
PDB entry 1buw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the S-nitroso form of liganded human hemoglobin.
Chan NL, Rogers PH, Arnone A
Biochemistry 37 16459-64 (1998)
PMID: 9843411

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 15.92 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand NO 4 x NO
1 modified residue:
Ligand SNC 2 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 97.1Å b: 101.2Å c: 61.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.244

Quick links

Citations

19 review citations

Chemical physiology of blood flow regulation by red blood cells: the role of nitric oxide and S-nitrosohemoglobin.
Singel et al. (2005)
18 more

9 mentions without citation

NO and Heme Proteins: Cross-Talk between Heme and Cysteine Residues.
Verde et al. (2023)
8 more