› 1bwl

X-ray diffraction
2.7Å resolution

OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H

Released:
DOI: 10.2210/pdb1bwl/pdb
PDB entry 1bwl coloured by chain, front view.
PDB entry 1bwl coloured by chain, side view.
PDB entry 1bwl coloured by chain, top view.
Source organism: Candida albicans
Primary publication:
On the active site of Old Yellow Enzyme. Role of histidine 191 and asparagine 194.
Brown BJ, Deng Z, Karplus PA, Massey V
J Biol Chem 273 32753-62 (1998)
PMID: 9830019

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 44.9 KDa
Source organism: Candida albicans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02899 (Residues: 2-399; Coverage: 100%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 143.6Å b: 143.6Å c: 42.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.273
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Applications of protein engineering to members of the old yellow enzyme family.
Amato et al. (2015)
2 more