› 1bxb

X-ray diffraction
2.2Å resolution

XYLOSE ISOMERASE FROM THERMUS THERMOPHILUS

Released:
DOI: 10.2210/pdb1bxb/pdb
PDB entry 1bxb coloured by chain, front view.
PDB entry 1bxb coloured by chain, side view.
PDB entry 1bxb coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structures of thermostable xylose isomerases from Thermus caldophilus and Thermus thermophilus: possible structural determinants of thermostability.
Chang C, Park BC, Lee DS, Suh SW
J Mol Biol 288 623-34 (1999)
PMID: 10329168

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 387 amino acids
Theoretical weight: 43.97 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: P26997 (Residues: 1-387; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 73.34Å b: 144.05Å c: 155.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Tagatose: properties, applications, and biotechnological processes.
Oh DK. (2007)
1 more

2 mentions without citation

On the evolutionary conservation of hydrogen bonds made by buried polar amino acids: the hidden joists, braces and trusses of protein architecture.
Worth et al. (2010)
1 more