› 1bye

X-ray diffraction
2.8Å resolution

GLUTATHIONE S-TRANSFERASE I FROM MAIS IN COMPLEX WITH ATRAZINE GLUTATHIONE CONJUGATE

Released:
DOI: 10.2210/pdb1bye/pdb
PDB entry 1bye coloured by chain, front view.
PDB entry 1bye coloured by chain, side view.
PDB entry 1bye coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Structures of herbicides in complex with their detoxifying enzyme glutathione S-transferase - explanations for the selectivity of the enzyme in plants.
Prade L, Huber R, Bieseler B
Structure 6 1445-52 (1998)
PMID: 9817846

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-146291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 213 amino acids
Theoretical weight: 23.72 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P12653 (Residues: 2-214; Coverage: 100%)
Gene name: GST1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand ATA 4 x ATA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 169.61Å b: 60.28Å c: 121.43Å
α: 90° β: 126.22° γ: 90°
R-values:
R R work R free
0.221 0.221 0.305
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily.
Sheehan et al. (2001)
3 more

4 mentions without citation

Plant glutathione transferases.
Dixon et al. (2002)
3 more