› 1bzy

X-ray diffraction
2Å resolution

HUMAN HGPRTASE WITH TRANSITION STATE INHIBITOR

Released:
DOI: 10.2210/pdb1bzy/pdb
PDB entry 1bzy coloured by chain, front view.
PDB entry 1bzy coloured by chain, side view.
PDB entry 1bzy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 2.0 A structure of human hypoxanthine-guanine phosphoribosyltransferase in complex with a transition-state analog inhibitor.
Shi W, Li CM, Tyler PC, Furneaux RH, Grubmeyer C, Schramm VL, Almo SC
Nat Struct Biol 6 588-93 (1999)
PMID: 10360366

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00492 (Residues: 2-218; Coverage: 100%)
Gene names: HPRT, HPRT1
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand MG 8 x MG
Ligand IMU 4 x IMU
Ligand POP 4 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 52.96Å b: 102.01Å c: 144.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Glycosidase mechanisms.
Rye et al. (2000)
7 more

20 mentions without citation

Genotype-phenotype correlations in neurogenetics: Lesch-Nyhan disease as a model disorder.
Fu et al. (2014)
19 more