› 1c0e

X-ray diffraction
2.2Å resolution

Active Site S19A Mutant of Bovine Heart Phosphotyrosyl Phosphatase

Released:
DOI: 10.2210/pdb1c0e/pdb
PDB entry 1c0e coloured by chain, front view.
PDB entry 1c0e coloured by chain, side view.
PDB entry 1c0e coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The structure of the bovine protein tyrosine phosphatase dimer reveals a potential self-regulation mechanism.
Tabernero L, Evans BN, Tishmack PA, Van Etten RL, Stauffacher CV
Biochemistry 38 11651-8 (1999)
PMID: 10512620

Function and Biology Details

Reactions catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight phosphotyrosine protein phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 17.93 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P11064 (Residues: 2-158; Coverage: 99%)
Gene name: ACP1
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 43.32Å b: 55.47Å c: 131.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.244
Expression system: Escherichia coli

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Citations

4 review citations

Protein tyrosine phosphatases: structure-function relationships.
Tabernero et al. (2008)
3 more