› 1c0f

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF DICTYOSTELIUM CAATP-ACTIN IN COMPLEX WITH GELSOLIN SEGMENT 1

Released:
DOI: 10.2210/pdb1c0f/pdb
PDB entry 1c0f coloured by chain, front view.
PDB entry 1c0f coloured by chain, side view.
PDB entry 1c0f coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the higher Ca(2+)-activation of the regulated actin-activated myosin ATPase observed with Dictyostelium/Tetrahymena actin chimeras.
Matsuura Y, Stewart M, Kawamoto M, Kamiya N, Saeki K, Yasunaga T, Wakabayashi T
J Mol Biol 296 579-95 (2000)
PMID: 10669610

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gelsolin Chain: S
Molecule details ›
Chain: S
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 53-176; Coverage: 16%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Major actin Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 40.96 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P07830 (Residues: 2-376; Coverage: 98%)
Gene names: DDB_G0269234, DDB_G0272248, DDB_G0272520, DDB_G0274129, DDB_G0274133, DDB_G0274135, DDB_G0274137, DDB_G0274285, DDB_G0274561, DDB_G0274599, DDB_G0274601, DDB_G0274727, DDB_G0280545, DDB_G0288879, DDB_G0289005, DDB_G0289553, DDB_G0289663, act1, act11, act12, act13, act14, act15, act16, act19, act1a, act2, act2-1, act20, act21, act4, act5, act6, act7, act8, act9, actA1, actA8, actB1, actM6
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 57Å b: 69.55Å c: 184.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.231
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Vertebrate tropomyosin: distribution, properties and function.
Perry SV. (2001)
3 more

8 mentions without citation

Structural basis for the slow dynamics of the actin filament pointed end.
Narita et al. (2011)
7 more