› 1c3o

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT MUTANT C269S WITH BOUND GLUTAMINE

Released:
DOI: 10.2210/pdb1c3o/pdb
PDB entry 1c3o coloured by chain, front view.
PDB entry 1c3o coloured by chain, side view.
PDB entry 1c3o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway.
Thoden JB, Huang X, Raushel FM, Holden HM
Biochemistry 38 16158-66 (1999)
PMID: 10587438

Function and Biology Details

Reactions catalysed: 
(1a) ATP + HCO(3)(-) = ADP + carboxyphosphate
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133743 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carbamoyl phosphate synthase large chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 1073 amino acids
Theoretical weight: 117.98 KDa
Source organism: Escherichia coli
Expression system: Bacteria
UniProt:
  • Canonical: P00968 (Residues: 1-1073; Coverage: 100%)
Gene names: JW0031, b0033, carB, pyrA
Sequence domains:
Structure domains:
Carbamoyl phosphate synthase small chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 382 amino acids
Theoretical weight: 41.47 KDa
Source organism: Escherichia coli
Expression system: Bacteria
UniProt:
  • Canonical: P0A6F1 (Residues: 1-382; Coverage: 100%)
Gene names: JW0030, b0032, carA, pyrA
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand MN 12 x MN
Ligand K 32 x K
Ligand CL 16 x CL
Ligand PO4 5 x PO4
Ligand ADP 8 x ADP
Ligand ORN 4 x ORN
Ligand GLN 8 x GLN
Ligand NET 4 x NET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 152.5Å b: 164.4Å c: 332.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.258
Expression system: Bacteria

Quick links

Citations

6 review citations

Genes, enzymes and regulation of arginine biosynthesis in plants.
Slocum RD. (2005)
5 more

7 mentions without citation

Deciphering CAD: Structure and function of a mega-enzymatic pyrimidine factory in health and disease.
Del Caño-Ochoa et al. (2021)
6 more