› 1c4f

X-ray diffraction
2.25Å resolution

GREEN FLUORESCENT PROTEIN S65T AT PH 4.6

Released:
DOI: 10.2210/pdb1c4f/pdb
PDB entry 1c4f coloured by chain, front view.
PDB entry 1c4f coloured by chain, side view.
PDB entry 1c4f coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural and spectral response of green fluorescent protein variants to changes in pH.
Elsliger MA, Wachter RM, Hanson GT, Kallio K, Remington SJ
Biochemistry 38 5296-301 (1999)
PMID: 10220315

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.95 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P212121
Unit cell:
a: 51.934Å b: 62.898Å c: 69.172Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 not available
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Creating new fluorescent probes for cell biology.
Zhang et al. (2002)
27 more

5 mentions without citation

Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
Stepanenko et al. (2008)
4 more