› 1c82

X-ray diffraction
1.7Å resolution

MECHANISM OF HYALURONAN BINDING AND DEGRADATION: STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE IN COMPLEX WITH HYALURONIC ACID DISACCHARIDE AT 1.7 A RESOLUTION

Released:
DOI: 10.2210/pdb1c82/pdb
PDB entry 1c82 coloured by chain, front view.
PDB entry 1c82 coloured by chain, side view.
PDB entry 1c82 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Mechanism of hyaluronan binding and degradation: structure of Streptococcus pneumoniae hyaluronate lyase in complex with hyaluronic acid disaccharide at 1.7 A resolution.
Ponnuraj K, Jedrzejas MJ
J Mol Biol 299 885-95 (2000)
PMID: 10843845

Function and Biology Details

Reaction catalysed: 
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hyaluronate lyase Chain: A
Molecule details ›
Chain: A
Length: 731 amino acids
Theoretical weight: 83.56 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q54873 (Residues: 285-1009; Coverage: 70%)
Gene name: SP_0314
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GCD
Carbohydrate polymer
2 bound ligands:
Ligand CAC 2 x CAC
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 83.55Å b: 104.19Å c: 99.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.257
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Hyaluronidases: their genomics, structures, and mechanisms of action.
Stern et al. (2006)
12 more

2 mentions without citation

Atomic-Resolution Experimental Structural Biology and Molecular Dynamics Simulations of Hyaluronan and Its Complexes.
Guvench O. (2022)
1 more