› 1c9s

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES

Released:
DOI: 10.2210/pdb1c9s/pdb
PDB entry 1c9s coloured by chain, front view.
PDB entry 1c9s coloured by chain, side view.
PDB entry 1c9s coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA.
Antson AA, Dodson EJ, Dodson G, Greaves RB, Chen X, Gollnick P
Nature 401 235-42 (1999)
PMID: 10499579

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo undecamer
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194932 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription attenuation protein MtrB Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V
Length: 74 amino acids
Theoretical weight: 8.26 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X6J6 (Residues: 1-74; Coverage: 100%)
Gene name: mtrB
Sequence domains: Tryptophan RNA-binding attenuator protein
Structure domains: TRAP-like
SINGLE STRANDED RNA (55-MER) Chain: W
Molecule details ›
Chain: W
Length: 55 nucleotides
Theoretical weight: 18.16 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRP 23 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 150.97Å b: 111.67Å c: 138.68Å
α: 90° β: 117.77° γ: 90°
R-values:
R R work R free
0.23 0.188 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

27 review citations

Ion mobility mass spectrometry of proteins and protein assemblies.
Uetrecht et al. (2010)
26 more

16 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
15 more