› 1c9u

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE IN COMPLEX WITH PQQ

Released:
DOI: 10.2210/pdb1c9u/pdb
PDB entry 1c9u coloured by chain, front view.
PDB entry 1c9u coloured by chain, side view.
PDB entry 1c9u coloured by chain, top view.
Source organism: Acinetobacter calcoaceticus
Primary publication:
Structure and mechanism of soluble quinoprotein glucose dehydrogenase.
Oubrie A, Rozeboom HJ, Kalk KH, Olsthoorn AJ, Duine JA, Dijkstra BW
EMBO J 18 5187-94 (1999)
PMID: 10508152

Function and Biology Details

Reaction catalysed: 
D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinoprotein glucose dehydrogenase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 50.29 KDa
Source organism: Acinetobacter calcoaceticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13650 (Residues: 25-478; Coverage: 100%)
Gene name: gdhB
Sequence domains: Glucose / Sorbosone dehydrogenase
Structure domains: TolB, C-terminal domain

Ligands and Environments


Cofactor: Ligand PQQ 2 x PQQ
2 bound ligands:
Ligand CA 6 x CA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: C2221
Unit cell:
a: 60.59Å b: 158.72Å c: 221.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.286
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The quinoprotein dehydrogenases for methanol and glucose.
Anthony C. (2004)
14 more

4 mentions without citation

Conformational changes in redox pairs of protein structures.
Fan et al. (2009)
3 more