› 1cbo

X-ray diffraction
1.8Å resolution

CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447ASN MUTANT

Released:
DOI: 10.2210/pdb1cbo/pdb
PDB entry 1cbo coloured by chain, front view.
PDB entry 1cbo coloured by chain, side view.
PDB entry 1cbo coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants.
Yue QK, Kass IJ, Sampson NS, Vrielink A
Biochemistry 38 4277-86 (1999)
PMID: 10194345

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.95 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 51.58Å b: 74.15Å c: 63.74Å
α: 90° β: 105.08° γ: 90°
R-values:
R R work R free
0.159 0.159 0.194
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Sequence-structure analysis of FAD-containing proteins.
Dym et al. (2001)
11 more

1 mention without citation

A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an Antarctic soil metagenomic library.
Bartasun et al. (2013)